Scientist– Mammalian Protein Expression and Molecular Biology (Non-PhD)

Pfizer - La Jolla, CA4.2

Full-time
Job Summary:
BioMedicine Design (BMD) is seeking a highly motivated scientist to join a protein expression and molecular biology group supporting the Biotherapeutics portfolio of Pfizer, Inc. in La Jolla, CA. Responsibilities will include execution on multiple steps of protein expression for biotherapeutic leads, protein reagents, immunogens, and panning reagents, as well as the design and building of DNA constructs. This includes strong expertise in mammalian tissue culture techniques using various cell lines and cell culture technologies. The ideal candidate will be highly proficient in the design of optimal processes that are consistent with team material needs for quantity (milligram to gram scale) and purity.

Job Description:
Perform and/or coordinate the generation of DNA constructs tailored for the expression of recombinant proteins
Perform protein expression in mammalian transient and stable cell lines, from various shake flask scales to bioreactor systems
Design, assess and troubleshoot cell culture and expression protocols.
Deliver cell culture supernatants and data analysis packages to project teams in a timely manner.
Present results regularly at team meetings
Perform diligent and timely notekeeping and work process tracking

Required Qualifications:
BA/BS in Biochemistry, Biology, or related field with 6+ years of R&D experience in an industry setting, or a Masters degree with 5+ years of industry experience
Extensive experience in protein expression using transient and/or stable mammalian cell culture systems
Strong expertise in design, execution, and troubleshooting of expression processes.
Expertise in techniques for the monitoring of cell culture density, viability and protein titer
Experience with antibody and biotherapeutic expression and a working knowledge of antibody molecules
Experience with data analysis and presentation software
Excellent written and oral communication skills
The ability to work closely with other group members within teams is essential
The individual should be scientifically creative and driven by a willingness to embrace innovation within drug discovery and an eagerness to challenge and meet aggressive timelines

Preferred Qualifications:
Expertise in the generation and design of plasmid constructs for expression of recombinant proteins, using cutting-edge molecular biology techniques
Experience with Insect Cell expression systems is a plus
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EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Other Job Details:
Eligible for Employee Referral Bonus
Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.