Working under the general direction of the Principal Investigator, the Research Technician II performs independently and efficiently to support a laboratory’s research endeavors and assists Instructors and Principal Investigators in a research laboratory. Performs or assists in experiments collaboratively and independently under minimum supervision of the project head and the PI of the laboratory. This includes collection of and accurate recording of procedures and results, maintenance of databases used in the lab, ordering necessary reagents to conduct experiments, and participation in joint upkeep of the lab.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Carries out a wide range of moderate to highly complex lab techniques such as, but not limited to, cell culture (primary and established cell lines), antibody production in vitro and in vivo, purification and determining concentration and activity, assays for cell proliferation and apoptosis and western blotting,
Performs small animal work related to antibody production in vivo and establishes and completes passages of patient -derived xenograft (PDX) Immunohistochemistry (IHC) staining .
Collects and processes clinical blood and tissue samples and maintains a tissue bank and its database. Collects and processes primary patient tumor or blood samples and establishes patient tissue and blood bank database.
Ships antibodies or cell samples or distributes them internally and receives antibody, drug or cell samples and makes detailed record for each “out” or “in” samples.
Participates in the design and/or modification of experiments, performs procedures and documents detailed protocols (i.e. cell and tissue culture, other routine assays and buffer preparation).
Notifies and seeks assistance from senior lab personnel or supervisor for any questions, unusual experimental conditions or results or needed training.
Participates in the development of new investigative techniques and/or determination of the most appropriate direction of research.
Maintains a lab notebook; collects and records study data. Prepares written weekly reports of completed, ongoing and future tasks, status of research, determination of technical procedures, sequence of operations, including statistical and graphic summaries of findings, interpretation of results and possible conclusions.
Collaborates with supervisor in organizing, writing and editing material for publication and may appear as secondary author on publications. Prepares and presents reports for lab meetings and journal clubs.
Performs independent literature searches.
Operates moderate to complex lab equipment such as spectrophotometer, electron microscope, scintillation center, ultra-centrifuges and other scientific equipment related to research activity. May perform, or refer as needed, routine maintenance of lab equipment to include cleaning and replacing of parts. Reports equipment malfunctions and needs to management.
Participates in the upkeep of the lab; this includes keeping both direct and general work spaces clean and tidy and helping maintain an overall safe and clean environment as well as keeping the laboratory organized (cell database, ascites database and purified antibody database, LN2 freezers, -80C freezers and -20 freezers).
On behalf of the principal investigator, distributes duty lists to lab members.
Maintains laboratory inventory and orders supplies,
Works safely within the lab environment and in performing work duties. Wears personal protective equipment as required.
May provide functional guidance and assistance to lower level technical personnel.
May teach moderately difficult to complex lab techniques to students and research personnel
Performs other duties as assigned.
SKILLS & COMPETENCIES REQUIRED:
Understanding and application of scientific research principals. Knowledge of basic science research and applicable field(s) of science, such as biology and chemistry. Knowledge and application of research protocols, including those related to small animal surgery.
Knowledge and skill to properly and safely use routine lab equipment and supplies.
Must be self-motivated, able to work independently and as a team player. Able to learn new techniques, procedures and equipment. Able to work carefully, accurately, efficiently and strictly follow instructions and/or protocols.
Must be highly organized to include good record-keeping, maintenance of laboratory research notebooks, and able to keep daily task/work log sheet and send weekly reports as required to document accomplished tasks, ongoing tasks, problems and work plans for the upcoming week.
Very good time-management skills. Must be able to complete assigned jobs within a given timeline.
Analytical skills and ability to resolve technical problems.
Proficiency in mathematics.
Strong communications skills to work effectively with the supervisor and others, including providing updates to the supervisor immediately if the given job cannot be done timely and the ability to communicate scientific and technical information. Proficiency in English (oral and written).
Must be able to work safely and properly use protective equipment
Computer literacy with some working knowledge of data management programs; some skill in Word, PowerPoint, Excel and internet searches. Data entry skills to maintain databases.
LICENSES, CERTIFICATIONS, and/or REGISTRATIONS:
Education: Bachelors Degree Required, Undergraduate degree in biology or biology related major
One year or more of directly related research laboratory experience required
Prudent use of hospital resources
Duties will be performed in a research laboratory. This setting may create contact or exposure to one or more of the following: chemicals, fluids, sharp instruments and other supplies and equipment consistent with a research lab. Contact or exposure may be airborne or physical. Standing, walking and sitting with occasional lifting, pushing and carrying required.
Work schedule may be flexible and may require evening, weekend or holiday hours.
The information contained in this document is intended to describe the general contents and requirements of work being performed by people assigned to this classification. It is not intended to be construed as an exhaustive statement of all duties, responsibilities or skills of individuals so classified.
: MA-Boston-MGH Main Campus
MGH Main Campus
55 Fruit Street
: Massachusetts General Hospital(MGH)
Standard Hours : 40
: Day Job
Recruiting Department : MGH Surgical Oncology Research
: May 23, 2018