Under the general supervision of the Mycobacteriology Mycology Group Manager, and with coordination with the Tuberculosis Diagnostics Team Leader, performs moderately complex to complex microbiological tasks. Examines and interprets liquid and solid mycobacterial culture media, evaluates prior testing results, and determines course-of-action. Maintains patient and culture records. Performs microscopic examination of clinical specimens and culture isolates. Processes clinical specimens for the isolation of acid-fast bacilli (AFB - Mycobacterium tuberculosis and related bacteria) and fungal organisms. Harvests positive cultures and saponifies mycolic acids from samples for identification by mycolic acid high performance liquid chromatography. Maintains the necessary equipment, reagents, and supplies for testing. Conducts, evaluates, qualifies, and documents Team personnel training on technical and support tasks as assigned. Participates in weekend rotation duties including reading equipment time and temperature performance, staining slides and autoclaving testing materials if needed. Completes other duties as assigned. Duties performed with adherence to standard protocol but with considerable use of scientific knowledge, initiative, and judgment
Essential Job Functions:
EJF 1. Under the technical guidance of the Team Leader, processes clinical specimens for the isolation of acid-fast bacilli (AFB - Mycobacterium tuberculosis and related bacteria) and fungal organisms. Decontaminates nonsterile specimens by NALC-sodium hydroxide method; concentrates specimens by centrifugation. Inoculates concentrated specimens to liquid and solid primary isolation media; prepares smears of concentrated specimens for staining while making no inoculation mistakes and having no evidence of cross-contamiation. Inoculates mycology specimens to primary isolation media. Trains others to perform these tests. Harvests positive cultures and saponifies mycolic acids for identification by mycolic acid high performance liquid chromatography. Duties performed on a rotational basis. (25%) EJF 2. Under the technical guidance of the Team Leader, performs microscopic examination of clinical specimens and culture isolates. Stains, examines, interprets, and reports AFB from clinical specimens by direct fluorochrome smear microscopy based on AFB morphology, fluorescence, and quantity. When necessary, overstains the direct fluorochrome smear to examine the smear microscopically using the Ziehl Neelsen or Kinyoun's staining procedure. Performs microscopic examination and interpretation of organisms from culture media by Ziehl Neelsen stain. Trains others to perform the staining procedures. Duties performed on a rotational basis. (25%) EJF 3. Maintains the necessary equipment, reagents, and supplies for testing. Completes appropriate log sheets by assigned deadlines. Performs quality control, quality assurance, equipment preventative maintenance and repair procedures; maintains written documentation of service and repairs performed as well as written and email documentation of work requests. Trains others to perform support tasks. (20%) EJF 4.Examines and interprets liquid and solid mycobacterial culture media, evaluates prior testing results, and determines course-of-action. Determines organism morphology and isolates organism(s) from culture media and from mixed cultures; subcultures to appropriate media. Consults with the senior microbiologist and Team Leader regarding problem or unusual cultures. Duties performed on a rotational basis.(15%) EJF 5. Maintains patient and sample records. Orders laboratory information system (LabWare) tests as needed for identification. Creates batches and completes appropriate log sheets by daily deadlines. Enters test results into LabWare by daily deadline. Trains others to perform demographics checks using LabWare demographic log or batch worksheets. Consults with internal and external customers, documents communications in phone and LabWare communication logs. (10%) EJF 6. Participates in weekend and holiday rotation duties including reading equipment time and temperature performance. Completes other duties as assigned. Other duties as assigned include but are not limited to actively participating and/or serving in a supporting role to meet the agency's obligations for disaster response and/or recovery or continuity of operations (COOP) activation. Such participation may require an alternate shift pattern assignment and/or location. (5%)
Knowledge Skills Abilities:
Knowledge of medical bacteriology, mycobacteriology, mycology, and biochemistry. Knowledge of laboratory safety, aseptic technique, and handling of pathogenic bacteria and fungi. Knowledge of quality assurance and quality control functions. Ability to complete tasks and meet deadlines without close supervision. Ability to communicate effectively orally and in writing; follow written and verbal instructions. Ability to manipulate specimens and cultures within a biological safety cabinet with speed, accuracy, and safety. Ability to use various items of laboratory equipment, including use of a computer for word processing, spreadsheet and database analysis and results reporting. Ability to interact effectively with staff, work independently, work in harmony with other team members, and follow standard operating procedures. Skill in manipulating standard laboratory equipment with speed, accuracy and safety. Skill to use brightfield, fluorescent, and dissecting microscope to examine slides and cultures.
Registration or Licensure Requirements:
- Have an earned bachelor’s, master’s, or doctoral degree in a chemical, physical, biological or clinical laboratory science, or medical technology from an accredited institution or - Have an earned associate’s degree in a chemical, physical, or biological science or medical laboratory technology from an accredited institution. - Experience and education may be substituted as defined by the Clinical Laboratory Improvement Amendments of 1988 (CLIA ’88) Standards.
Initial Selection Criteria:
Preferred but not required: Experience in a clinical microbiology laboratory testing specimens Experience in a clinical microbiology laboratory working with bacterial or fungal cultures Experience working with aseptic technique under a biological safety cabinet with clinical specimens Experience working with aseptic technique under a biological safety cabinet with microbiological or cell cultures Experience with brightfield and/or fluorescence microscopy examining prepared slides Experience with culture media interpretation Completion of college coursework in clinical laboratory science, public health bacteriology, and/or medical microbiology as documented on application or transcripts
Requisition 381624 Note: Military occupation(s) that relate to the initial selection criteria and registration or licensure requirements for this position may include: 67B, 71A, 230X, 43TX. For more information see the Texas State Auditor’s Military Crosswalk at http://www.hr.sao.state.tx.us/Compensation/JobDescriptions.aspx. Applicant is required to list all college level biology, microbiology, chemistry and other science courses on application or a separate sheet showing the course name and number of semester hours (copy of college transcript is acceptable). Applicant may fax list of completed coursework as described above to AccessHR at 1-866-245-3646. Please note Requisition number on all faxed information. Applicants with diplomas awarded in countries other than the U.S. may locate an approved credentialing agency at this link https://tea.texas.gov/Texas_Educators/Certification/Out-of-State_Certification/Foreign_Credential_Evaluation_Services/ and must provide credentialing agency foreign transcripts assessment report with this application. Must be willing to work on a weekend and holiday scheduled rotation. Must be able to wear provided personal protective equipment (including: N95 respirator or powered air purifying respirator [PAPR], safety glasses or face shield, gloves, laboratory coat) as required by testing/safety procedures. Must be able to handle highly infectious agents such as Mycobacterium tuberculosis. Moderate risk to tuberculosis. Annual tuberculosis skin tests administered by Safety Officer. Must be able to pass test for color-blindness.
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