My lab develops and applies new tools for imaging the brain in animal models with cellular and subcellular resolution. We are particularly interested in genetically encoded voltage indicators (GEVIs) which are biosensors based on fluorescent proteins that enable non-invasive monitoring of neural electrical activity by imaging rather than by traditional electrophysiological methods. These indicators have already been applied in Drosophila and in rodent brain slices, but need further improvement to be of broad utility in mammalian in vivo models. We are seeking a new team member to lead our efforts to characterize new probes we are developing by combined electrophysiology and imaging in vitro, in brain slices, and in vivo. An ideal candidate is someone who has experience developing new systems, either electrophysiology rigs and/or custom microscopes. Programming experience in Matlab or other languages is essential for customizing microscope control and data analysis. An ideal candidate would enjoy working in an energetic team-based environment and work efficiently. An ideal candidate can also adapt quickly to new challenges as the project evolves.
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Conducting research projects, both independently and with others
Purchasing, setting up and maintaining equipment necessary to conduct research
Teaching techniques, procedures, and experimental design to other members of the lab
Communicating research results during our group meetings, and in local/national/international research meetings and conferences.
Master's degree in a Basic Science. Experience in lieu of degree will not be accepted.
Directly related experience, typically as a Research Assistant, Research Technician or Postdoctoral Fellow/Associate.
Ability to set up electrophysiology rigs and/or custom microscopes
Coding proficiency, ideally in Matlab
Ability to prepare brain slices.
Ability to express transgenes in neurons of brain slices using viruses and/or in utero electroporation.
Whole-cell patch clamping of neurons in brain slices. Additional experience with neurons in dissociated cultures is preferable.
Experience with imaging neural activity (e.g. voltage or calcium) in brain slices or in vivo, using 1P and/or 2P imaging
Experience building electrical/electronic/electromechanical devices.