University of Washington - Seattle, WA4.1

Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here.
As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. All of which has allowed the UW to be nationally recognized as a “Great College to Work For” for four consecutive years.

The University of Washington, Department of Pathology is a broad and dynamic department that strives for a consistent level of high achievement in all of its activities. The Department offers full diagnostic services in all areas of Pathology, is a top ranked research organization as measured by research awards from the National Institutes of Health, and offers an array of specialized educational training programs.

UW Medicine Pathology brings together state-of-the-art technology and highly trained, world renowned personnel to provide comprehensive diagnostic and personalized service to physicians, community hospitals, independent laboratories, and other health care organizations. Our operations are based at the University of Washington Medical Center, Harborview Medical Center, South Lake Union, Northwest Hospital and the Seattle Cancer Care Alliance. Pathology faculty members also serve at Seattle Children's Hospital, the Veteran’s Administration Puget Sound Healthcare System, and the Fred Hutchinson Cancer Research Center. These collaborative efforts have generated innovative, translational studies in the basic mechanisms of disease as they apply to diagnostic pathology.

The Kennedy lab has an outstanding opportunity for a part-time 70% position as a Research Scientist/Engineer 2 to perform experiments related to early cancer detection. The research will focus on developing next-generation sequencing methods to detect low frequency cancer-associated DNA mutations from blood, urine, and tissue samples as a diagnostic and prognostic marker.

Job duties:
Prepare DNA samples for sequencing using established protocols; Maintain and organize data/records.
Routine use of PCR machines; requiring sequencing protocols.
Perform quantitative PCR
Perform solution-based DNA hybridizations
Perform experiments designed to optimize and extend novel sequencing methods being developed in the lab.
Collate and organize data for monthly, quarterly, semi-annual, and annual grant reports
Support lab operations through compliance maintenance; certifications, paperwork maintaining equipment and ordering reagents

Minimum Requirements:
Bachelors degree in Molecular/Cellular Biology/Biology or relevant field.
Minimum of two years of experience in a related field or equivalent
Be able to program protocols in to quantitative qPCR machines.
Be familiar basic biochemical and enzymatic manipulations of DNA, such as purification, restriction enzyme digestion, gel electrophoresis, etc.
Experience with safe handling of biohazardous materials, such as human cells and tissue samples.
Working knowledge of next generation sequencing methods, equipment, and data manipulation.
Be able to read and understand relevant peer-reviewed literature.

Having 2 or more years of relevant laboratory experience
Experience with performing DNA sequencing library preparation protocols for the Illumina Next-Generation Sequencing platform.
Be familiar with high accuracy sequencing methods on next-generation sequencing platforms
Demonstrated ability think critically and perform experiments independently