Research Technician - GENIE

Howard Hughes Medical Institute - Ashburn, VA (30+ days ago)4.2


Summary:
Scientists at Janelia Research Campus are engaged in exciting, multidisciplinary projects in neurobiology and imaging. The Genetically-Encoded Neuronal Indicator and Effector (GENIE) project produces and optimizes cutting-edge fluorescent protein-based sensors for neural activity imaging for high-resolution monitoring of brain function. The GENIE project employs project team members with expertise in advanced molecular cloning, protein engineering, high-throughput cell-based screening, in vivo imaging, and image analysis. This Research Technician will conduct in vitro, mammalian cell-based screens to improve sensor functionality; and manufacture, purify, and biophysically characterize fluorescent protein-based sensors.

Essential Duties and Responsibilities:
DNA transfection of tissue culture cells
High-throughput bacterial library screening assays
High-throughput mammalian cell-based screening assays
Protein biochemistry and biophysical characterization of fluorescent protein-based sensors
Set-up, operate, and maintain laboratory instruments
Record and communicate data in laboratory notebooks, written reports, and oral presentations
Report the work done and the methods used to the Project Scientist
Contribute to team meetings and planning

Scope:
This position has no budgetary responsibility
This position has no supervisory responsibility

Preferred Qualifications:
Education / Experience:
Bachelor’s degree in the life sciences
Expert level cell biologist with greater than 5 years of laboratory experience
Protein biochemistry experience
Molecular biology experience

Skills and Abilities:
Perform complex motor tasks with speed and precision handling small volumes of reagents
Safely and accurately perform laboratory bench work for up to 8 hours per day
Operate microscopes
Operate and program liquid handling robotics
Focus attention on a repetitive task for extended periods
Excellent oral and written communication skills
Keep accurate and organized records of work performed

Physical Requirements:
Regularly reaches and grasps with arms and hands
Regularly uses fine motor skills to pipette and manipulate small objects
Long periods of standing and/or sitting

HHMI is an Equal Opportunity Employer.

Required Skills

Required Experience