The Department of Biochemistry and Molecular Biology (BMB) at the University of Chicago seeks a cryo-electron microscopist. A Staff Scientist is responsible for all aspects of research projects and research facilities. Plans and conducts research; facilitates and monitors daily activities of research projects. Directs engineering and technical support activities to develop and maintain tools and computational methods needed to gather and analyze data.
The individual will develop and support research and operations in electron microscopy (EM) focused on high-resolution single particle cryo-electron microscopy (cryo-EM).
BMB, together with the University of Chicago Electron Microscopy Facility has EM resources located at the Gordon Center for Integrated Sciences. EM instrumentation at the facility includes a ThermoFisher Talos 200 kV FEG-TEM, an FEI Spirit 120 kV TEM, and an FEI F30300 kV TEM.
Additional preparative equipment includes a ThermoFisher Vitrobot Mark IV, a Gatan CP3, a Gatan Solara plasma-cleaners and carbon evaporators, a Baltec HPM-010 high-pressure freezer, a Leica cryo-ultramicrotome, and a freeze substitution device. These will be complemented by the upcoming installation of a Titan Krios with a K3 Bioquantum direct electron detector with STEM/TEM tomography capabilities.
The facility will support research from investigators in BMB with established strengths in structural biology, as well as related areas of biophysics, biochemistry and cell biology.
The position requires independent judgment in the application of cryo-EM in research in structural biology.
The individual will assist in the operation and maintenance of the cryo-electron microscopes and associated equipment.
Tasks include setting up experiments and assisting users as prescribed by a supervisor (includes grid making and ice thickness optimization).
The individual will assist users in image processing and other computational tasks associated with the generation of high-resolution structures from the single particle data. Some flexibility will be allowed to carry out independent research projects and as part of this research, submission of relevant research grants.
The individual will maintain records, files and logs of work performed in laboratory notebooks and computer databases and will compile data and record results.
The individual will work with the EM Core facility Director and Scientific Director to perform related tasks as required.
Education, Experience or Certifications:
Ph. D. in biology, biochemistry, biophysics, physics or related fields required.
Two year’s experience in electron microscopy required.
Two years of post-doctoral required, post-doctoral experience in membrane protein structural biology preferred.
NOTE: When applying, all required documents MUST be uploaded under the Resume/CV section of the application
Depends on Qualifications
Scheduled Weekly Hours
Job is Exempt?
Drug Test Required?
Does this position require incumbent to operate a vehicle on the job?
Health Screen Required?
Remove from Posting On or Before