A RESEARCH TECHNICIAN position is open in the laboratory of Dr. Rudolph Tanzi within the Genetics and Aging Research Unit at the Massachusetts General Hospital and Harvard Medical School. The long-term goal of the project is to use the understanding of CD33 function to design novel therapeutic strategies for enhancing clearance of the aggregation-prone amyloid beta peptide in Alzheimer's disease. The aims of the project are to study the effect of CD33 on amyloid beta uptake and clearance by microglial cells, secretion of pro-inflammatory cytokines by microglia, and activation state of microglial cells. The project will include experiments using cell and tissue culture approach (mostly immortalized microglial cell lines). The effects of CD33 on amyloid beta clearance in cell culture will be analyzed by using BCA protein assay, SDS-PAGE, Western blotting, enzyme-linked immunosorbent assay (ELISA), as well as fluorescence microscopy. Experiments will occasionally include histopathological analysis and assessment of amyloid beta in the mouse brain tissue by using ELISA assays.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Please note, the functions below are representative of major duties that are typically associated with these positions. Specific responsibilities may vary based upon departmental needs. Similarly, not all duties that have been outlined will be assigned to each position.
Prepares basic solutions and performs base-level procedures as assigned (i.e. – pipetting, cell and tissue culture, etc.)
Maintains laboratory notebook
Understands and applies basic scientific techniques
Conducts analysis of results and may begin interpretation of results
Sets up and prepares routine experiments as directed
Prepares lab reagents, chemicals, instruments and equipment
May perform independent literature searches
Assists with organizing materials for publication or presentation
Maintains and orders supplies
A Research Technician II performs the duties of a Research Technician I (above) and may also:
Execute protocols of non-routine experiments
Assist the PI(s) with determining the most suitable methodology
Perform basic design and modification of protocols
Calculate, transcribe and analyze data
Prepare and present reports
Organize and summarize acquired data, using scientific and statistical techniques.
Organize and/or draft material for the preparation of research papers, manuscripts and other documents for publication and/or presentation.
Participate in the design of experiments or field work.
SKILLS/ABILITIES/COMPETENCIES REQUIRED :
Ability to work independently and as a team member
Good communication skills
Working knowledge of clinical research protocols
Ability to perform multiple tasks independently
The Research Technician II should also possess:
Analytical skills and the ability to resolve technical problems
Ability to interpret acceptability of data results
Working knowledge of data management programs
Demonstrated competence in research techniques and methodologies
Bachelor’s degree required.
New graduates with some lab experience (via course work, internships, etc.) or those without any prior research experience will be considered for the Research Technician I position outlined above.
Those with a minimum of 1 year of directly related work experience will be considered for a Research Technician II position.
SUPERVISORY RESPONSIBILITY (if applicable):
A Research Technician II may serve as a team leader to lab assistants and Research Technician I’s.
Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.
: MA-Charlestown-529 Main - MGH
529 Main - MGH
529 Main Street
: Massachusetts General Hospital(MGH)
Standard Hours : 40
: Day Job
Recruiting Department : MGH Neurology
: Aug 3, 2018