The Laboratory Assistant will work under the close supervision of the Research Scientist or PI at the Lewis Katz’s School of Medicine’s Shriners Pediatric Research Center. The Laboratory Assistant will perform PCR-based genotyping and maintenance of genetically engineered mice for a neuroscience research lab. The Laboratory Assistant will study the molecular and cellular mechanisms underlying axon regeneration failure and neuronal damage and aim to develop the effective therapeutic strategies for repairing CNS injuries and disorders, including spinal cord trauma, optic nerve injury and multiple sclerosis. The incumbent will keep the relevant information in a MS Excel file so that all related projects will be planned in a timely manner. Responsibilities include performing in vitro experiments, such as cell cultures, molecular biology techniques (DNA preparation and cloning), and biochemical experiments; mouse colony handling and management; conducting in vivo animal experiments, partial surgical procedures in rodents, postsurgical animal care and treatment, and behavioral tests; and assisting with histological techniques such as processing of brain and spinal cord, embedding, dissection, sectioning and immunostaining. In addition, the incumbent will assist with experimental tasks such as medicinal i.p. injections into mice, maintaining lab equipment; compiling and organizing research data and routine lab material ordering. Performs other duties as assigned.
Required Education and Experience:
At least one year of related laboratory experience. An equivalent combination of education and experience may be considered.
Required Skills and Abilities:
- Demonstrated ability to manage mouse/rodent breeding colonies and perform mouse/rodent handling functions.
- Demonstrated ability to perform histological techniques such as tissue embedding, sectioning and staining.
- Demonstrated computer skills, and proficiency with spreadsheet and database software programs.
- Demonstrated knowledge of PCR and basic molecular biology method(s).
- Demonstrated organizational skills with the ability to handle multiple tasks.
- Ability to work in an environment exposed to fumes, airborne particles, and laboratory mice without an allergic reaction.
- Demonstrated ability to complete experiments in a timely-manner.
- Demonstrated ability to operate standard laboratory equipment.
- Demonstrated written and verbal communication skills.
- Excellent communication and interpersonal skills.
Bachelor’s degree in Science.
This is a grant funded position.
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In the performance of their functions as detailed in the position description employees have an obligation to avoid ethical, legal, financial and other conflicts of interest to ensure that their actions and outside activities do not conflict with their primary employment responsibilities at the institution. Employees are also expected to understand and be in compliance with applicable laws, University and. employment policies and regulations, including NCAA regulations for areas and departments which their essential functions cause them to interact.
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