POSITION OBJECTIVE
Working under limited supervision, the Research Assistant 3 will carry out complex small molecule discovery projects and validations in the Cardiac Therapeutics Laboratory. This position will use various molecular methods to bridge the gap of understanding between structure and experimental observations in cardiac muscle physiology. This research assistant contributes to multiple projects in the lab that are funded by NIH grants. This position will work with animals.
ESSENTIAL FUNCTIONS
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Coordinate research activities including a wide array of cellular and molecular biology experiments using high throughput screening, cell-based assays, gel electrophoresis, western blots, and other in vitro techniques. Design, develop, and advance biochemical and cell-based high throughput screen-compatible assays for small molecule screening. (30%)
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Work closely with the principal investigator and collaborate with scientists to adapt benchtop assays to automated workflows utilizing automated equipment available in core facilities. May supervise students and technicians. (10%)
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Devise new protocols and techniques for research projects involving a high degree of skill and training. Execute high throughput screening (HTS) campaigns using multiple molecule libraries and analyze large, complex datasets to identify and prioritize hit compounds. (20%)
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Execute and optimize bioassays to assess the potency of hit compounds in-vitro and in-vivo. (20%)
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Evaluate adequacy of techniques; study and test new procedures and analyze, interpret, and clearly document experimental data, observations, and conclusions. (8%)
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Prepare and present research results in lab meetings. May co-author research projects. (6%)
NONESSENTIAL FUNCTIONS
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May monitor the lab budget. Maintain equipment, prepare buffer/media/reagents, and dispose of biohazardous waste. (3%)
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Perform other duties assigned. (3%)
CONTACTS
Department: Weekly contact with principal investigator and lab members to discuss research, maintain workflow, goals, lab operation/management issues, ordering, and reimbursement etc. Frequent contact with laboratory members in collaboration in common research project.
University: Occasional interaction with other faculty in the department and the university to provide updates on common collaborative research projects. Utilize core university facilities for experimentation and maintain strong relationships with core managers for research collaborations.
External: Occasional communication with research collaborators from other institutions to exchange information.
Students: Occasional contact with students who shadow/observe the research.
SUPERVISORY RESPONSIBILITY
No direct supervisory responsibilities. May supervise students and technicians.
QUALIFICATIONS
Experience: 3 to 5 years of related experience required.
Education: Bachelor of Science degree required.
REQUIRED SKILLS
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Knowledge of commonly used concepts, practices and procedures in chemistry, physics, biochemistry and molecular/cellular biology and in vivo cardiovascular function.
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Previous experience working with animals preferred.
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Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to the Animal Resource Center¿s mission. Must handle animals with care and respect at all times.
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Experience in cell-based and/or biochemical assays.
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Proficiency in basic molecular biology, cell biology and biochemistry methods.
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Ability to perform delicate manual tasks requiring fine motor skills while working with samples.
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Ability to follow instructions and pre-established guidelines to perform the functions of the job with minimal supervision.
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Strong organization skills and maintain a laboratory notebook, attention to scientific detail.
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Ability to coordinate multiple projects, prioritize tasks, and maintain data integrity.
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Ability to effectively operate basic molecular and cellular biology laboratory equipment.
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Ability to problem-solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision-making.
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Ability to work effectively, both independently and collaboratively within a team.
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Ability to learn and apply new techniques, ideas, processes, policies, functions, etc. Must be highly motivated and a self-starter.
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Ability to conform to shifting priorities, demands and timelines. Must be flexible in order to respond to project adjustments and alterations promptly and efficiently. Must be able to adapt to varying workloads and respond appropriately to stressful situations.
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Excellent verbal and written communication skills; good interpersonal skills with the ability to interact with colleagues and supervisors face to face.
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Ability to demonstrate successful support, education, and advocacy for all students, aligned with the values, mission, and messaging of the university, while adhering to the staff policy on conflict of commitment and interest.
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Willingness to learn and work with Artificial Intelligence (AI) tools and technologies.
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Ability to follow through on assigned projects and provide timely follow-up as needed.
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Ability to meet consistent attendance.
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Must demonstrate compliance with university animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
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Must be able and willing to learn new techniques, procedures, processes, and computer gear to protect the health of the animals.
WORKING CONDITIONS
Working conditions will be standard laboratory environment with associated hazards, such as chemical and biohazards. Employee can expect to be working on a bench with molecular reagents and/or cardiac tissues, working with animal models/and bacterial cells in the laboratory, working in front of a computer for several hours, working outside standard working days or hours as required. The employee will work with frequent interruptions and distractions. The employee will need to take Chemical Safety Training and Biohazard Material Training. Other training may be required. Required personal protective equipment may include a standard lab coat, gloves and safety glasses when appropriate.
Case Western Reserve University's animal facilities are accredited by the Associate for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the ¿Guide for the Care and Use of Laboratory Animals¿ appropriate Federal Animal Welfare Regulations, and the Public Health Service ¿Policy on the Humane Care and Use of Laboratory Animals.¿ This position and all animal research personnel are subject to internal compliance to the School of Medicine's Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.
