The candidate will join our fast paced research group in protein biochemistry and recombinant DNA technology. The position entails producing and expressing recombinant proteins to support crystallographic efforts for co-crystal structure determination with clients’ compounds and our fragment library.
The ideal candidate will have the following background/skill sets:
- Ph.D., or Masters, in molecular biology, biochemistry, or the equivalent knowledge and experience, is required.
- Must be highly organized, motivated and able to work independently in a collaborative team-oriented setting
- Excellent communication and organizational skills are a must.
- Minimum of 3 years experience in the following areas:
- Cloning including PCR, restriction analysis, DNA sequencing, primer and vector design of clones for use in E. coli and/or baculovirus expression systems. Experienceith Baculovirus cell cultures (including Wave bioreactor fermentations) and transfection techniques a plus
- Protein expression analysis, optimization, and scale-up using bioreactors
- Design and execute protein purification and characterization protocols to obtain the best protein for the project
- Protein purification of recombinant proteins including but not limited to: His-tag, GST-tag, Maltose affinity purification, Ion Exchange, Hydrophobic Interaction, Size Exclusion
- Protein refolding
- Purification of untagged proteins
- Western blotting and SDS-PAGE techniques
- Assay Development: Fluorescence, TRF, Luminescence, PF, Couple assay
- Working knowledge of Bioinformatics and databases would be helpful
