Position Summary/ Department Summary:
Research Assistant II and Lab Manager for the Laboratory of Joseph Majzoub, MD, Division of Endocrinology, Department of Pediatrics, Boston Children’s Hospital.
Key Responsibilities:-
Maintain multiple mouse colonies including breeding, genotyping, with accurate records. This is one of the most important roles
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Maintain records of experimental methodologies and results in a log of work performed (Lab Archives online notebook).
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Work with PI and independently to design and perform experiments
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Perform a variety of routine laboratory experiments and procedures, utilizing standard laboratory equipment, recording and analyzing outcomes for completeness and accuracy.
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Mouse necropsy and tissue processing
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Mouse tissue histological analysis using light microscopy, immunofluorescence, RNA in situ hybridization (RNAscope)
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ELISA, RIA, qRTPCR
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Cell culture ideal but not required
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Maintain equipment, lab area and supplies in a neat manner.
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Process orders for supplies and animals
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Assist PI to write and amend animal protocols
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Collaborate with other members of the Majzoub lab and with other laboratories in the Endocrinology department
Minimum Qualifications
Education:
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Bachelor’s degree in biology, neuroscience, or related field
Experience:-
At least 1 year experience managing laboratory mouse colonies
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Preferred experience with PCR, ELISA, RIA, immunocytochemistry, RNAscope
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Good problem-solving skills and independent work ability
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Excellent organizational and communication skills
