The Department of Neurology is seeking a full-time Research Assistant (Life Science Research Professional 1) to support, manage and facilitate the molecular-related activities in the lab whose interest is in elucidating the role of glia in normal brain plasticity and the pathophysiology of glioma (malignant glia). The LSRP1 will work under general guideance of the Principal Investigator and post-doctoral fellows to assist in research using molecular techniques. Prior experience with isolation/culture of neural cell types, design and validation of molecular constructs for genetic modification of cell lines, and single-cell sequencing is preferred.
The lab’s research program focuses on gliomas such as glioblastoma and diffuse intrinsic pontine glioma (DIPG) and the microenvironmental determinants of normal and malignant neural precursor cell behavior. Microglial influences on normal neural precursor cell function and the impact of microglial neuroinflammation following cancer therapies on cellular mechanisms of neuroplasticity and brain homeostasis is one area of deep focus (Monje et al, 2002, Nature Medicine, Monje et al, 2003, Science, Monje et al, 2007, Annals of Neurology, Gibson et al., 2019 Cell). The regulation of normal and malignant glial cells by neuronal activity is another area of particular emphasis (Gibson et al., 2014, Science; Venkatesh et al., 2015, Cell) and the research group is working to decipher and therapeutically target the molecular mechanisms that underlie neuronal interactions with gliomas (Venkatesh et al, 2017, Nature). In addition to these studies of mature neurons in the glioma microenvironment, we also study the influences of neural precursor cells on glioma (Qin et al., 2017, Cell). Together with these basic studies, the research program executes preclinical studies of novel therapeutics for high-grade gliomas (Grasso et al, 2015, Nature Medicine; Nagaraja et al, 2017, Cancer Cell; Mount et al, 2018 Nature Medicine) in order to translate new therapies to the clinic for glial cancers.
Duties include*:
- Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory.
- Independently conduct experiments; maintain detailed records of experiments and outcomes.
- Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
- Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
- Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals.
- Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
- Assist with orientation and training of new staff or students on lab procedures or techniques.
*- Other duties may also be assigned.
Qualifications:
DESIRED QUALIFICATIONS:
- Bachelor's degree in related scientific field and three years of relevant work experience; or Master’s degree with two years of relevant work experience.
- Knowledge and experience with FACs analysis, PCR, culture of human and mouse cells, Western blotting, ELISA assays, cloning, single cell RNA sequencing, CRISPR/CAS9 editing constructs.
Excellent documentation skills.
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EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
- Thorough understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
- Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
- Ability to maintain detailed records of experiments and outcomes.
- General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
- Ability to work under deadlines with general guidance.
Excellent organizational skills and demonstrated ability to accurately complete detailed work.
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PHYSICAL REQUIREMENTS*:
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
WORKING CONDITIONS:
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
May require extended or unusual work hours based on research requirements and business needs.
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WORK STANDARDS:
- Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
- Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors bases on training and lessons learned.
Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University’s Administrative Guide, http://adminguide.stanford.edu .
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The expected pay range for this position is $32.58 to $38.48 per hour. Stanford University provides pay ranges representing its good faith estimate of the salary or hourly wage the university reasonably expects to pay for a position upon hire. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location and external market pay for comparable jobs.
At Stanford University, base pay represents only one aspect of the comprehensive rewards package. The Cardinal at Work website ( https://cardinalatwork.stanford.edu/benefits-rewards ) provides detailed information on Stanfordʼs extensive range of benefits and rewards offered to employees. Specifics about the rewards package for this position may be discussed during the hiring process.
Consistent with its obligations under the law, the University will provide reasonable accommodations to applicants and employees with disabilities. Applicants requiring a reasonable accommodation for any part of the application or hiring process should contact Stanford University Human Resources by submitting a contact form .
Stanford is an equal employment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law.
The job duties listed are typical examples of work performed by positions in this job classification and are not designed to contain or be interpreted as a comprehensive inventory of all duties, tasks, and responsibilities. Specific duties and responsibilities may vary depending on department or program needs without changing the general nature and scope of the job or level of responsibility. Employees may also perform other duties as assigned.
